Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol.
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ABSTRACT:
SUBMITTER: Milani C
PROVIDER: S-EPMC3711900 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol.
Milani Christian C Hevia Arancha A Foroni Elena E Duranti Sabrina S Turroni Francesca F Lugli Gabriele Andrea GA Sanchez Borja B Martín Rebeca R Gueimonde Miguel M van Sinderen Douwe D Margolles Abelardo A Ventura Marco M
PloS one 20130715 7
Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as ...[more]
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