Unknown

Dataset Information

0

Galleria mellonella larvae as an infection model for group A streptococcus.


ABSTRACT: Group A streptococcus is a strict human pathogen that can cause a wide range of diseases, such as tonsillitis, impetigo, necrotizing fasciitis, toxic shock, and acute rheumatic fever. Modeling human diseases in animals is complicated, and rapid, simple, and cost-effective in vivo models of GAS infection are clearly lacking. Recently, the use of non-mammalian models to model human disease is starting to re-attract attention. Galleria mellonella larvae, also known as wax worms, have been investigated for modeling a number of bacterial pathogens, and have been shown to be a useful model to study pathogenesis of the M3 serotype of GAS. In this study we provide further evidence of the validity of the wax worm model by testing different GAS M-types, as well as investigating the effect of bacterial growth phase and incubation temperature on GAS virulence in this model. In contrast to previous studies, we show that the M-protein, among others, is an important virulence factor that can be effectively modeled in the wax worm. We also highlight the need for a more in-depth investigation of the effects of experimental design and wax worm supply before we can properly vindicate the wax worm model for studying GAS pathogenesis.

SUBMITTER: Loh JM 

PROVIDER: S-EPMC3714134 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Galleria mellonella larvae as an infection model for group A streptococcus.

Loh Jacelyn M S JM   Adenwalla Nazneen N   Wiles Siouxsie S   Proft Thomas T  

Virulence 20130507 5


Group A streptococcus is a strict human pathogen that can cause a wide range of diseases, such as tonsillitis, impetigo, necrotizing fasciitis, toxic shock, and acute rheumatic fever. Modeling human diseases in animals is complicated, and rapid, simple, and cost-effective in vivo models of GAS infection are clearly lacking. Recently, the use of non-mammalian models to model human disease is starting to re-attract attention. Galleria mellonella larvae, also known as wax worms, have been investiga  ...[more]

Similar Datasets

| S-EPMC3524148 | biostudies-literature
| S-EPMC6463115 | biostudies-literature
| S-EPMC6813211 | biostudies-literature
| S-EPMC4920769 | biostudies-literature
| S-EPMC6086298 | biostudies-literature
| S-EPMC7526850 | biostudies-literature
| S-EPMC5801645 | biostudies-literature