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Severe soft tissue infection caused by a non-beta-hemolytic Streptococcus pyogenes strain harboring a premature stop mutation in the sagC gene.

ABSTRACT: We recovered a non-beta-hemolytic Streptococcus pyogenes strain from a severe soft tissue infection. In this isolate, we detected a premature stop codon within the sagC gene of the streptolysin S (SLS) biosynthetic operon. Reintroduction of full-length sagC gene on a plasmid vector restored the beta-hemolytic phenotype to our clinical isolate, indicating that the point mutation in sagC accounted for loss of hemolytic activity. To the best of our knowledge, this is the first report to demonstrate that a severe soft tissue infection can be caused by a non-beta-hemolytic S. pyogenes strain lacking a functional SagC.

SUBMITTER: Jantsch J

PROVIDER: S-EPMC3716101 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature

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Severe soft tissue infection caused by a non-beta-hemolytic Streptococcus pyogenes strain harboring a premature stop mutation in the sagC gene.

Jantsch Jonathan J Gerlach Roman G RG Ensser Armin A Dahesh Samira S Popp Isabel I Heeg Christiane C Bleiziffer Oliver O Merz Thomas T Schulz Theresia T Horch Raymund E RE Bogdan Christian C Nizet Victor V van der Linden Mark M

Journal of clinical microbiology 20130320 6

We recovered a non-beta-hemolytic Streptococcus pyogenes strain from a severe soft tissue infection. In this isolate, we detected a premature stop codon within the sagC gene of the streptolysin S (SLS) biosynthetic operon. Reintroduction of full-length sagC gene on a plasmid vector restored the beta-hemolytic phenotype to our clinical isolate, indicating that the point mutation in sagC accounted for loss of hemolytic activity. To the best of our knowledge, this is the first report to demonstrate ...[more]

PMID: 23515542

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