Ontology highlight
ABSTRACT:
SUBMITTER: Chen C
PROVIDER: S-EPMC3718762 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Chen Changmin C Wonsey Diane R DR Lemieux Madeleine E ME Kung Andrew L AL
PloS one 20130722 7
Fusion of the EWS gene to FLI1 produces a fusion oncoprotein that drives an aberrant gene expression program responsible for the development of Ewing sarcoma. We used a homogenous proximity assay to screen for compounds that disrupt the binding of EWS-FLI1 to its cognate DNA targets. A number of DNA-binding chemotherapeutic agents were found to non-specifically disrupt protein binding to DNA. In contrast, actinomycin D was found to preferentially disrupt EWS-FLI1 binding by comparison to p53 bin ...[more]