Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes.
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ABSTRACT:
SUBMITTER: Shin SC
PROVIDER: S-EPMC3720884 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Advantages of Single-Molecule Real-Time Sequencing in High-GC Content Genomes.
Shin Seung Chul SC Ahn Do Hwan DH Kim Su Jin SJ Lee Hyoungseok H Oh Tae-Jin TJ Lee Jong Eun JE Park Hyun H
PloS one 20130723 7
Next-generation sequencing has become the most widely used sequencing technology in genomics research, but it has inherent drawbacks when dealing with high-GC content genomes. Recently, single-molecule real-time sequencing technology (SMRT) was introduced as a third-generation sequencing strategy to compensate for this drawback. Here, we report that the unbiased and longer read length of SMRT sequencing markedly improved genome assembly with high GC content via gap filling and repeat resolution. ...[more]
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