Ontology highlight
ABSTRACT:
SUBMITTER: Tan YJ
PROVIDER: S-EPMC3721342 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Tan Yu-Jing YJ Sui Dexin D Wang Wei-Han WH Kuo Min-Hao MH Reid Gavin E GE Bruening Merlin L ML
Analytical chemistry 20130528 12
Selective enrichment of phosphopeptides prior to their analysis by mass spectrometry (MS) is vital for identifying protein phosphorylation sites involved in cellular regulation. This study describes modification of porous nylon substrates with TiO2 nanoparticles to create membranes that rapidly enrich phosphopeptides. Membranes with a 22-mm diameter bind 540 nmol of phosphoangiotensin and recover 70% of the phosphopeptides in mixtures with a 15-fold excess of nonphosphorylated proteins. Recovery ...[more]