Trypan blue staining to determine vaginal exposure in two types of plastic vaginal applicators containing two different microbicide formulations.
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ABSTRACT:
SUBMITTER: Hemmerling A
PROVIDER: S-EPMC3726043 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
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Trypan blue staining to determine vaginal exposure in two types of plastic vaginal applicators containing two different microbicide formulations.
Hemmerling Anke A Harrison William G WG Brown Joelle Morgan JM Moscicki Barbara B Oziemkowska Maria M Bukusi Elizabeth A EA Cohen Craig R CR
Sexually transmitted diseases 20120901 9
Dye staining of applicators has been shown to be a reliable and objective method to test vaginal insertion in clinical microbicide trials, but different plastics, dyes, and product formulations may impact the accuracy of this method. Reportedly used applicators returned from 3 clinical trials were stained with 1% trypan blue. In a phase 1 study (VivaGel), using gel-filled HTI polypropylene applicators, 1271 (97%) of applicators stained positive. In a phase 1 and a phase 2a study (LACTIN-V) using ...[more]
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