Dataset Information

Characterization of Cannabis sativa allergens.

ABSTRACT:

Background

Allergic sensitization to Cannabis sativa is rarely reported, but the increasing consumption of marijuana has resulted in an increase in the number of individuals who become sensitized. To date, little is known about the causal allergens associated with C sativa.

Objective

To characterize marijuana allergens in different components of the C sativa plant using serum IgE from marijuana sensitized patients.

Methods

Serum samples from 23 patients with a positive skin prick test result to a crude C sativa extract were evaluated. IgE reactivity was variable between patients and C sativa extracts. IgE reactivity to C sativa proteins in Western blots was heterogeneous and ranged from 10 to 70 kDa. Putative allergens derived from 2-dimensional gels were identified.

Results

Prominent IgE reactive bands included a 23-kDa oxygen-evolving enhancer protein 2 and a 50-kDa protein identified to be the photosynthetic enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase. Additional proteins were identified in the proteomic analysis, including those from adenosine triphosphate synthase, glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase, and luminal binding protein (heat shock protein 70), suggesting these proteins are potential allergens. Deglycosylation studies helped refine protein allergen identification and demonstrated significant IgE antibodies against plant oligosaccharides that could help explain cross-reactivity.

Conclusion

Identification and characterization of allergens from C sativa may be helpful in further understanding allergic sensitization to this plant species.

SUBMITTER: Nayak AP

PROVIDER: S-EPMC3726218 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Publications

Characterization of Cannabis sativa allergens.

Nayak Ajay P AP Green Brett J BJ Sussman Gordon G Berlin Noam N Lata Hemant H Chandra Suman S ElSohly Mahmoud A MA Hettick Justin M JM Beezhold Donald H DH

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 20130522 1

<h4>Background</h4>Allergic sensitization to Cannabis sativa is rarely reported, but the increasing consumption of marijuana has resulted in an increase in the number of individuals who become sensitized. To date, little is known about the causal allergens associated with C sativa.<h4>Objective</h4>To characterize marijuana allergens in different components of the C sativa plant using serum IgE from marijuana sensitized patients.<h4>Methods</h4>Serum samples from 23 patients with a positive skin ...[more]

PMID: 23806457

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Project description:BackgroundFor millennia, drug-type cannabis strains were extensively used for various medicinal, ritual, and inebriant applications. However, cannabis prohibition during the last century led to cultivation and breeding activities being conducted under clandestine conditions, while scientific development of the crop ceased. Recently, the potential of medicinal cannabis has been reacknowledged and the now expanding industry requires optimal and scientifically characterized varieties. However, scientific knowledge that can propel this advancement is sorely lacking. To address this issue, the current study aims to provide a better understanding of key physiological and phenological traits that can facilitate the breeding of advanced cultivars.ResultsA diverse population of 121 genotypes of high-THC or balanced THC-CBD ratio was cultivated under a controlled environment facility and 13 plant parameters were measured. No physiological association across genotypes attributed to the same vernacular classification was observed. Floral bud dry weight was found to be positively associated with plant height and stem diameter but not with days to maturation. Furthermore, the heritability of both plant height and days to maturation was relatively high, but for plant height it decreased during the vegetative growth phase. To advance breeding efficacy, a prediction equation for forecasting floral bud dry weight was generated, driven by parameters that can be detected during the vegetative growth phase solely.ConclusionsOur findings suggest that selection for taller and fast-growing genotypes is likely to lead to an increase in floral bud productivity. It was also found that the final plant height and stem diameter are determined by 5 independent factors that can be used to maximize productivity through cultivation adjustments. The proposed prediction equation can facilitate the selection of prolific genotypes without the completion of a full cultivation cycle. Future studies that will associate genome-wide variation with plants morphological traits and cannabinoid profile will enable precise and accelerated breeding through genomic selection approaches.

Dataset Information

Characterization of Cannabis sativa allergens.

Background

Objective

Methods

Results

Conclusion

Publications

Characterization of Cannabis sativa allergens.

Similar Datasets

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