Ebola virus VP35 induces high-level production of recombinant TPL-2-ABIN-2-NF-?B1 p105 complex in co-transfected HEK-293 cells.
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ABSTRACT:
SUBMITTER: Gantke T
PROVIDER: S-EPMC3727213 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
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Ebola virus VP35 induces high-level production of recombinant TPL-2-ABIN-2-NF-κB1 p105 complex in co-transfected HEK-293 cells.
Gantke Thorsten T Boussouf Sabrina S Janzen Julia J Morrice Nicholas A NA Howell Steven S Mühlberger Elke E Ley Steven C SC
The Biochemical journal 20130601 2
Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression locus 2)-ABIN-2 [A20-binding inhibitor of NF-κB (nuclear factor κB) 2]-NF-κB1 p105 complex. VP35 also ...[more]
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