Ontology highlight
ABSTRACT:
SUBMITTER: Seo A
PROVIDER: S-EPMC3728560 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Seo Angie A Jackson Janelle L JL Schuster Jolene V JV Vardar-Ulu Didem D
Analytical and bioanalytical chemistry 20130607 19
Many in-vitro experiments performed to study the response of thiol-containing proteins to changes in environmental redox potentials use dithiothreitol (DTT) to maintain a preset redox environment throughout the experiments. However, the gradual oxidation of DTT during the course of the experiments, and the interaction between DTT and other components in the system, can significantly alter the initial redox potential and complicate data interpretation. Having an internal reporter of the actual re ...[more]