Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.
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ABSTRACT:
SUBMITTER: Delsaute M
PROVIDER: S-EPMC3729153 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
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Three-dimensional structure of RBcel1, a metagenome-derived psychrotolerant family GH5 endoglucanase.
Delsaute Maud M Berlemont Renaud R Dehareng Dominique D Van Elder Dany D Galleni Moreno M Bauvois Cédric C
Acta crystallographica. Section F, Structural biology and crystallization communications 20130726 Pt 8
RBcel1 is an endoglucanase belonging to glycoside hydrolase family 5 subfamily 5 (GH5_5) that was recently identified from a soil metagenome library from the Antarctic. Unlike its closest structural homologue (Cel5A from Thermoascus aurantiacus), this enzyme was reported to be able to catalyze transglycosylation reactions and has putatively been implicated in the bacterial cellulose-synthesis process. Here, the structure of RBcel1 at 1.4 Å resolution, solved by molecular replacement, is reported ...[more]
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