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ABSTRACT:
SUBMITTER: Fujita J
PROVIDER: S-EPMC3729168 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Fujita Junso J Miyazaki Yuma Y Hirose Mika M Nagao Chioko C Mizohata Eiichi E Matsumoto Yoshimi Y Mizuguchi Kenji K Inoue Tsuyoshi T Matsumura Hiroyoshi H
Acta crystallographica. Section F, Structural biology and crystallization communications 20130727 Pt 8
FtsA from methicillin-resistant Staphylococcus aureus (MRSA) was cloned, overexpressed and purified. The protein was crystallized using the sitting-drop vapour-diffusion technique. A cocrystal with β-γ-imidoadenosine 5'-phosphate (AMPPNP; a nonhydrolysable ATP analogue) was grown using PEG 3350 as a precipitant at 293 K. X-ray diffraction data were collected to a resolution of 2.3 Å at 100 K. The crystal belonged to the monoclinic space group P2₁, with unit-cell parameters a = 75.31, b = 102.78, ...[more]