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Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.

ABSTRACT: Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His? tag diffracted to a resolution of 2.3 Å and belonged to space group P3?21, containing a single molecule in the asymmetric unit. The crystal of BcCITase bearing a C-terminal His6 tag diffracted to a resolution of 1.9 Å and belonged to space group P2?2?2?, containing two molecules in the asymmetric unit.

SUBMITTER: Suzuki N

PROVIDER: S-EPMC3729181 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic analysis of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040.

Suzuki Nobuhiro N Kim Young Min YM Momma Mitsuru M Fujimoto Zui Z Kobayashi Mikihiko M Kimura Atsuo A Funane Kazumi K

Acta crystallographica. Section F, Structural biology and crystallization communications 20130727 Pt 8

Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase (BcCITase) catalyses an intramolecular transglucosylation reaction and produces cycloisomaltooligosaccharides from dextran. BcCITase was overexpressed in Escherichia coli in two different forms and crystallized by the sitting-drop vapour-diffusion method. The crystal of BcCITase bearing an N-terminal His₆ tag diffracted to a resolution of 2.3 Å and belonged to space group P3₁21, containing a single molecule in the asymmetr ...[more]

PMID: 23908050

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