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Genome-wide profiling of human cap-independent translation-enhancing elements.

ABSTRACT: We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells.

SUBMITTER: Wellensiek BP

PROVIDER: S-EPMC3731418 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Genome-wide profiling of human cap-independent translation-enhancing elements.

Wellensiek Brian P BP Larsen Andrew C AC Stephens Bret B Kukurba Kim K Waern Karl K Briones Natalia N Liu Li L Snyder Michael M Jacobs Bertram L BL Kumar Sudhir S Chaput John C JC

Nature methods 20130616 8

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells ...[more]

PMID: 23770754

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