Ontology highlight
ABSTRACT:
SUBMITTER: Dubrovsky EB
PROVIDER: S-EPMC373292 | biostudies-literature | 2004
REPOSITORIES: biostudies-literature
Dubrovsky Edward B EB Dubrovskaya Veronica A VA Levinger Louis L Schiffer Steffen S Marchfelder Anita A
Nucleic acids research 20040109 1
Although correct tRNA 3' ends are crucial for protein biosynthesis, generation of mature tRNA 3' ends in eukaryotes is poorly understood and has so far only been investigated in vitro. We report here for the first time that eukaryotic tRNA 3' end maturation is catalysed by the endonuclease RNase Z in vivo. Silencing of the JhI-1 gene (RNase Z homolog) in vivo with RNAi in Drosophila S2 cultured cells causes accumulation of nuclear and mitochondrial pre-tRNAs, suggesting that JhI-1 encodes both f ...[more]