Computational design of orthogonal nucleoside kinases.
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ABSTRACT:
SUBMITTER: Liu L
PROVIDER: S-EPMC3733048 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
Computational design of orthogonal nucleoside kinases.
Liu Lingfeng L Murphy Paul P Baker David D Lutz Stefan S
Chemical communications (Cambridge, England) 20101019 46
We report the computational enzyme design of an orthogonal nucleoside analog kinase for 3'-deoxythymidine. The best kinase variant shows an 8500-fold change in substrate specificity, resulting from a 4.6-fold gain in catalytic efficiency for the nucleoside analog and a 2000-fold decline for the native substrate thymidine. ...[more]
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