Unlocking hidden genomic sequence.
Ontology highlight
ABSTRACT:
SUBMITTER: Keith JM
PROVIDER: S-EPMC373418 | biostudies-literature | 2004 Feb
REPOSITORIES: biostudies-literature
Publications
Unlocking hidden genomic sequence.
Keith Jonathan M JM Cochran Duncan A E DA Lala Gita H GH Adams Peter P Bryant Darryn D Mitchelson Keith R KR
Nucleic acids research 20040218 3
Despite the success of conventional Sanger sequencing, significant regions of many genomes still present major obstacles to sequencing. Here we propose a novel approach with the potential to alleviate a wide range of sequencing difficulties. The technique involves extracting target DNA sequence from variants generated by introduction of random mutations. The introduction of mutations does not destroy original sequence information, but distributes it amongst multiple variants. Some of these varia ...[more]
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