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Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.

ABSTRACT: We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld+Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld+Lo. Our studies start from two published ternary phase diagrams, and show how these can be combined into a quaternary phase diagram by study of a few hundred samples of intermediate compositions.

SUBMITTER: Konyakhina TM

PROVIDER: S-EPMC3738200 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Phase diagram of a 4-component lipid mixture: DSPC/DOPC/POPC/chol.

Konyakhina Tatyana M TM Wu Jing J Mastroianni James D JD Heberle Frederick A FA Feigenson Gerald W GW

Biochimica et biophysica acta 20130607 9

We report the first 4-component phase diagram for the lipid bilayer mixture, DSPC/DOPC/POPC/chol (distearoylphosphatidylcholine/dioleoylphosphatidylcholine/1-palmitoyl, 2-oleoylphosphatidylcholine/cholesterol). This phase diagram, which has macroscopic Ld+Lo phase domains, clearly shows that all phase boundaries determined for the 3-component mixture containing DOPC transition smoothly into the boundaries for the 3-component mixture containing POPC, which has nanoscopic phase domains of Ld+Lo. O ...[more]

PMID: 23747294

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