Dataset Information

NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca²?-mobilization/influx and cortisol secretion.

ABSTRACT: Purinergic receptor expression and involvement in steroidogenesis were examined in NCI-H295R (H295R), a human adrenal cortex cell line which expresses all the key enzymes necessary for steroidogenesis. mRNA/protein for multiple P1 (A(2A) and A(2B)), P2X (P2X? and P2X?), and P2Y (P2Y?, P2Y?, P2Y?, P2Y??, P2Y??, and P2Y??) purinergic receptors were detected in H295R. 2MeS-ATP (10-1000 µM), a P2Y? agonist, induced glucocorticoid (GC) secretion in a dose-dependent manner, while other extracellular purine/pyrimidine agonists (1-1000 µM) had no distinct effect on GC secretion. Extracellular purines, even non-steroidogenic ones, induced Ca²?-mobilization in the cells, independently of the extracellular Ca²? concentration. Increases in intracellular Ca²? concentration induced by extracellular purine agonists were transient, except when induced by ATP or 2MeS-ATP. Angiotensin II (AngII: 100 nM) and dibutyryl-cyclic AMP (db-cAMP: 500 µM) induced both GC secretion and Ca²?-mobilization in the presence of extracellular Ca²? (1.2 mM). GC secretion by AngII was reduced by nifedipine (10-100 µM); whereas the Ca²? channel blocker did not inhibit GC secretion by 2MeS-ATP. Thapsigargin followed by extracellular Ca²? exposure induced Ca²?-influx in H295R, and the cells expressed mRNA/protein of the component molecules for store-operated calcium entry (SOCE): transient receptor C (TRPC) channels, calcium release-activated calcium channel protein 1 (Orai-1), and the stromal interaction molecule 1 (STIM1). In P2Y?-knockdown, 2MeS-ATP-induced GC secretion was significantly inhibited. These results suggest that H295R expresses a functional P2Y? purinergic receptor for intracellular Ca²?-mobilization, and that P2Y? is linked to SOCE-activation, leading to Ca²?-influx which might be necessary for glucocorticoid secretion.

SUBMITTER: Nishi H

PROVIDER: S-EPMC3738630 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca²⁺-mobilization/influx and cortisol secretion.

Nishi Haruhisa H Arai Hirokazu H Momiyama Toshihiko T

PloS one 20130808 8

Purinergic receptor expression and involvement in steroidogenesis were examined in NCI-H295R (H295R), a human adrenal cortex cell line which expresses all the key enzymes necessary for steroidogenesis. mRNA/protein for multiple P1 (A(2A) and A(2B)), P2X (P2X₅ and P2X₇), and P2Y (P2Y₁, P2Y₂, P2Y₆, P2Y₁₂, P2Y₁₃, and P2Y₁₄) purinergic receptors were detected in H295R. 2MeS-ATP (10-1000 µM), a P2Y₁ agonist, induced glucocorticoid (GC) secretion in a dose-dependent manner, while other extracellular p ...[more]

PMID: 23951072

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Dataset Information

NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca²?-mobilization/influx and cortisol secretion.

Publications

NCI-H295R, a human adrenal cortex-derived cell line, expresses purinergic receptors linked to Ca²⁺-mobilization/influx and cortisol secretion.

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