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Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles.

ABSTRACT: We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.

SUBMITTER: Wang Z

PROVIDER: S-EPMC3740541 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles.

Wang Zongxing Z Wu Hung-jen HJ Fine Daniel D Schmulen Jeffrey J Hu Ye Y Godin Biana B Zhang John X J JX Liu Xuewu X

Lab on a chip 20130801 15

We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer. ...[more]

PMID: 23743667

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