Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.
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ABSTRACT:
SUBMITTER: Battisti A
PROVIDER: S-EPMC3742097 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Publications
Intracellular pH measurements made simple by fluorescent protein probes and the phasor approach to fluorescence lifetime imaging.
Battisti Antonella A Digman Michelle A MA Gratton Enrico E Storti Barbara B Beltram Fabio F Bizzarri Ranieri R
Chemical communications (Cambridge, England) 20120419 42
A versatile pH-dependent fluorescent protein was applied to intracellular pH measurements by means of the phasor approach to fluorescence lifetime imaging. By this fit-less method we obtain intracellular pH maps under resting or altered physiological conditions by single-photon confocal or two-photon microscopy. ...[more]
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