MicroRNA-27a is induced by leucine and contributes to leucine-induced proliferation promotion in C2C12 cells.
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ABSTRACT:
SUBMITTER: Chen X
PROVIDER: S-EPMC3742232 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Publications
MicroRNA-27a is induced by leucine and contributes to leucine-induced proliferation promotion in C2C12 cells.
Chen Xiaoling X Huang Zhiqing Z Chen Daiwen D Yang Ting T Liu Guangmang G
International journal of molecular sciences 20130708 7
Leucine, a branched chain amino acid, is well known to stimulate protein synthesis in skeletal muscle. However, the role of leucine in myoblast proliferation remains unclear. In this study, we found that leucine could promote proliferation of C2C12 cells. Moreover, expressions of miR-27a and myostatin (a bona fide target of miR-27a) were upregulated and downregulated, respectively, following leucine treatment. We also found that miR-27a loss-of-function by transfection of a miR-27a inhibitor sup ...[more]
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