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Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice.

ABSTRACT: BACKGROUND: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. OBJECTIVE: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. METHODS: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m(3). Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. RESULTS: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. CONCLUSION: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.

SUBMITTER: Su CL

PROVIDER: S-EPMC3742529 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice.

Su Chien-Ling CL Chen Tzu-Tao TT Chang Chih-Cheng CC Chuang Kai-Jen KJ Wu Cheng-Kuan CK Liu Wen-Te WT Ho Kin Fai KF Lee Kang-Yun KY Ho Shu-Chuan SC Tseng Hsiu-Er HE Chuang Hsiao-Chi HC Cheng Tsun-Jen TJ

International journal of nanomedicine 20130802

<h4>Background</h4>Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood.<h4>Objective</h4>The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches.<h4>Methods</h4>Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m(3). Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to deter ...[more]

PMID: 23946650

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Project description:Silver nanoparticles (AgNPs) have attracted much interest and have been used for antibacterial, antifungal, anticancer, and antiangiogenic applications because of their unique properties. The increased usage of AgNPs leads to a potential hazard to human health. However, the potential effects of AgNPs on animal models are not clear. This study was designed to investigate the potential impact of AgNPs on pregnant mice.The synthesis of AgNPs was performed using culture extracts of Bacillus cereus. The synthesized AgNPs were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, and transmission electron microscopy. AgNPs were administrated into pregnant mice via intravenous infusion at 1.0 mg/kg doses at 6.5 days postcoitum (dpc). At 13.5, 15.5, and 17.5 dpc, the pregnant mice were euthanized, and the embryo and placenta were isolated. The meiotic status of oocytes was evaluated. DNA methylation studies were performed, and aberrant imprinting disrupted fetal, placental, and postnatal development. Quantitative real-time polymerase chain reaction analysis and Western blot were used to analyze various gene expressions.The synthesized AgNPs were uniformly distributed and were spherical in shape with an average size of 8 nm. AgNPs exposure increased the meiotic progression of female germ cells in the fetal mouse ovaries, and maternal AgNP exposure significantly disrupted imprinted gene expression in 15.5 dpc embryos and placentas, such as Ascl2, Snrpn, Kcnq1ot1, Peg3, Zac1, H19, Igf2r, and Igf2; DNA methylation studies revealed that AgNPs exposure significantly altered the methylation levels of differentially methylated regions of Zac1.The results from this study indicated that early exposure to AgNPs has the potential to disrupt fetal and postnatal health through epigenetic changes in the embryo and abnormal development of the placenta. These results can contribute to research involved in the safe use of various biomedical applications of AgNPs and improves the understanding of the development of AgNPs in biomedical applications.

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Dataset Information

Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice.

Publications

Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice.

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