Anandamide-derived prostamide F2? negatively regulates adipogenesis.

ABSTRACT: Lipid mediators variedly affect adipocyte differentiation. Anandamide stimulates adipogenesis via CB1 receptors and peroxisome proliferator-activated receptor ?. Anandamide may be converted by PTGS2 (COX2) and prostaglandin F synthases, such as prostamide/prostaglandin F synthase, to prostaglandin F2? ethanolamide (PGF2?EA), of which bimatoprost is a potent synthetic analog. PGF2?EA/bimatoprost act via prostaglandin F2?FP receptor/FP alt4 splicing variant heterodimers. We investigated whether prostamide signaling occurs in preadipocytes and controls adipogenesis. Exposure of mouse 3T3-L1 or human preadipocytes to PGF2?EA/bimatoprost during early differentiation inhibits adipogenesis. PGF2?EA is produced from anandamide in preadipocytes and much less so in differentiating adipocytes, which express much less PTGS2, FP, and its alt4 splicing variant. Selective antagonism of PGF2?EA receptors counteracts prostamide effects on adipogenesis, as does inhibition of ERK1/2 phosphorylation. Selective inhibition of PGF2?EA versus prostaglandin F2? biosynthesis accelerates adipogenesis. PGF2?EA levels are reduced in the white adipose tissue of high fat diet-fed mice where there is a high requirement for new adipocytes. Prostamides also inhibit zebrafish larval adipogenesis in vivo. We propose that prostamide signaling in preadipocytes is a novel anandamide-derived antiadipogenic mechanism.

SUBMITTER: Silvestri C 

PROVIDER: S-EPMC3743501 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature