Ontology highlight
ABSTRACT:
SUBMITTER: Wang Y
PROVIDER: S-EPMC3744876 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Wang Yanli Y Ludwig Janos J Schuberth Christine C Goldeck Marion M Schlee Martin M Li Haitao H Juranek Stefan S Sheng Gang G Micura Ronald R Tuschl Thomas T Hartmann Gunther G Patel Dinshaw J DJ
Nature structural & molecular biology 20100627 7
RIG-I is a cytosolic helicase that senses 5'-ppp RNA contained in negative-strand RNA viruses and triggers innate antiviral immune responses. Calorimetric binding studies established that the RIG-I C-terminal regulatory domain (CTD) binds to blunt-end double-stranded 5'-ppp RNA a factor of 17 more tightly than to its single-stranded counterpart. Here we report on the crystal structure of RIG-I CTD bound to both blunt ends of a self-complementary 5'-ppp dsRNA 12-mer, with interactions involving 5 ...[more]