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Sequence selective recognition of double-stranded RNA at physiologically relevant conditions using PNA-peptide conjugates.

ABSTRACT: Conjugation of short peptide nucleic acids (PNA) with tetralysine peptides strongly enhanced triple helical binding to RNA at physiologically relevant conditions. The PNA hexamers and heptamers carrying cationic nucleobase and tetralysine modifications displayed high binding affinity for complementary double-stranded RNA without compromising sequence selectivity. The PNA-peptide conjugates had unique preference for binding double-stranded RNA, while having little, if any, affinity for double-stranded DNA. The cationic PNAs were efficiently taken up by HEK293 cells, whereas little uptake was observed for unmodified PNA.

SUBMITTER: Muse O

PROVIDER: S-EPMC3745792 | biostudies-literature | 2013 Aug

REPOSITORIES: biostudies-literature

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Sequence selective recognition of double-stranded RNA at physiologically relevant conditions using PNA-peptide conjugates.

Muse Oluwatoyosi O Zengeya Thomas T Mwaura Juddy J Hnedzko Dziyana D McGee Dennis W DW Grewer Christof T CT Rozners Eriks E

ACS chemical biology 20130612 8

Conjugation of short peptide nucleic acids (PNA) with tetralysine peptides strongly enhanced triple helical binding to RNA at physiologically relevant conditions. The PNA hexamers and heptamers carrying cationic nucleobase and tetralysine modifications displayed high binding affinity for complementary double-stranded RNA without compromising sequence selectivity. The PNA-peptide conjugates had unique preference for binding double-stranded RNA, while having little, if any, affinity for double-str ...[more]

PMID: 23721369

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