Augmenting transcriptome assembly by combining de novo and genome-guided tools.
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ABSTRACT:
SUBMITTER: Jain P
PROVIDER: S-EPMC3746961 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Augmenting transcriptome assembly by combining de novo and genome-guided tools.
Jain Prachi P Krishnan Neeraja M NM Panda Binay B
PeerJ 20130815
Researchers interested in studying and constructing transcriptomes, especially for non-model species, face the conundrum of choosing from a number of available de novo and genome-guided assemblers. None of the popular assembly tools in use today achieve requisite sensitivity, specificity or recovery of full-length transcripts on their own. Here, we present a comprehensive comparative study of the performance of various assemblers. Additionally, we present an approach to combinatorially augment t ...[more]
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