Identification and Characterization of Eurotium rubrum Isolated from Meju in Korea.

ABSTRACT: We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus is yellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at 25?. Cleistothecia are first bright yellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at 20?, 37 mm at 25? and 32 mm at 30? after 15 days. The isolate grew slower on 2% MEA (< 20 mm 15 days at 25?) compared to CYA and PDA (< 40 mm 15 days at 25?). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 40~75 µm in diameter. Asci are 8-spored and globose to subglobose 8~11 µm. Ascospores are disciform, 4.0~5.0 µm in length and 4.2~4.5 µm in width. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.6~6.0 µm in length and 3.0~4.3 µm in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin (750 bp) gene and 100% in ?-tubulin (1016 bp) gene. The E. rubrum isolate also had weak ?-glucosidase and protease activities.

SUBMITTER: Yun YH 

PROVIDER: S-EPMC3749414 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature