Combining high-throughput sequencing with fruit body surveys reveals contrasting life-history strategies in fungi.

ABSTRACT: Before the recent revolution in molecular biology, field studies on fungal communities were mostly confined to fruit bodies, whereas mycelial interactions were studied in the laboratory. Here we combine high-throughput sequencing with a fruit body inventory to study simultaneously mycelial and fruit body occurrences in a community of fungi inhabiting dead wood of Norway spruce. We studied mycelial occurrence by extracting DNA from wood samples followed by 454-sequencing of the ITS1 and ITS2 regions and an automated procedure for species identification. In total, we detected 198 species as mycelia and 137 species as fruit bodies. The correlation between mycelial and fruit body occurrences was high for the majority of the species, suggesting that high-throughput sequencing can successfully c

SUBMITTER: Ovaskainen O 

PROVIDER: S-EPMC3749500 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature