Ontology highlight
ABSTRACT:
SUBMITTER: Donald RGK
PROVIDER: S-EPMC3749728 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Donald Robert G K RGK Flint Mike M Kalyan Narender N Johnson Erik E Witko Susan E SE Kotash Cheryl C Zhao Ping P Megati Shakuntala S Yurgelonis Irina I Lee Phillip Kwok PK Matsuka Yury V YV Severina Elena E Deatly Anne A Sidhu Mini M Jansen Kathrin U KU Minton Nigel P NP Anderson Annaliesa S AS
Microbiology (Reading, England) 20130429 Pt 7
The Clostridium difficile toxins A and B are primarily responsible for symptoms of C. difficile associated disease and are prime targets for vaccine development. We describe a plasmid-based system for the production of genetically modified toxins in a non-sporulating strain of C. difficile that lacks the toxin genes tcdA and tcdB. TcdA and TcdB mutations targeting established glucosyltransferase cytotoxicity determinants were introduced into recombinant plasmids and episomally expressed toxin mu ...[more]