Systematic identification of signal-activated stochastic gene regulation.
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ABSTRACT:
SUBMITTER: Neuert G
PROVIDER: S-EPMC3751578 | biostudies-literature | 2013 Feb
REPOSITORIES: biostudies-literature
Publications
Systematic identification of signal-activated stochastic gene regulation.
Neuert Gregor G Munsky Brian B Tan Rui Zhen RZ Teytelman Leonid L Khammash Mustafa M van Oudenaarden Alexander A
Science (New York, N.Y.) 20130201 6119
Although much has been done to elucidate the biochemistry of signal transduction and gene regulatory pathways, it remains difficult to understand or predict quantitative responses. We integrate single-cell experiments with stochastic analyses, to identify predictive models of transcriptional dynamics for the osmotic stress response pathway in Saccharomyces cerevisiae. We generate models with varying complexity and use parameter estimation and cross-validation analyses to select the most predicti ...[more]
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