Suberoylanilide hydroxamic acid induces limited changes in the transcriptome of primary CD4(+) T cells.

ABSTRACT:

Objective

To assess the off-target effects of the histone deacetylase inhibitor (HDACi) suberoylanilide hydroxamic acid (SAHA) in human primary CD4 T cells.

Design

A pharmacologically relevant concentration (340 nmol/l) of SAHA was shown to significantly increase histone hyperacetylation by 24 h and this length of treatment was selected to determine its impact on gene expression in primary CD4 T cells.

Methods

Illumina Beadchips for microarray gene expression analysis were used to analyze differential gene expression between cells treated or not with SAHA with a paired analysis using multivariate permutation tests. Gene ontology, biological pathway and protein interaction network analyses were used to identify the higher order biological processes affected by SAHA tre

SUBMITTER: Beliakova-Bethell N 

PROVIDER: S-EPMC3752851 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature