Analysis of repetitive DNA in chromosomes by flow cytometry.
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ABSTRACT:
SUBMITTER: Brind'Amour J
PROVIDER: S-EPMC3753228 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Analysis of repetitive DNA in chromosomes by flow cytometry.
Brind'Amour Julie J Lansdorp Peter M PM
Nature methods 20110501 6
We developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (FISH), called CFF, to analyze repetitive DNA in chromosomes using FISH with directly labeled peptide nucleic acid (PNA) probes. We used CFF to measure the abundance of interstitial telomeric sequences in Chinese hamster chromosomes and major satellite sequences in mouse chromosomes. Using CFF we also identified parental homologs of human chromosome 18 with different amounts of repetitive DNA. ...[more]
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