Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay.
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ABSTRACT:
SUBMITTER: Feng X
PROVIDER: S-EPMC3754660 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Rapid differentiation of cryptic species within Cryptococcus gattii by a duplex PCR assay.
Feng Xiaobo X Fu Xiaohua X Ling Bo B Wang Lei L Liao Wanqing W Pan Weihua W Yao Zhirong Z
Journal of clinical microbiology 20130703 9
Cryptococcus gattii consists of four cryptic species, VGI, VGII, VGIII, and VGIV. Herein, a duplex PCR assay using two primer pairs targeting the vacuolar membrane gene and the intergenic spacer region was developed. It successfully distinguished the cryptic species according to the distinct size of the amplicons. ...[more]
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