Ontology highlight
ABSTRACT:
SUBMITTER: Chiodo F
PROVIDER: S-EPMC3754922 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Chiodo Fabrizio F Marradi Marco M Tefsen Boris B Snippe Harm H van Die Irma I Penadés Soledad S
PloS one 20130827 8
Improved detection of anti-carbohydrate antibodies is a need in clinical identification of biomarkers for cancer cells or pathogens. Here, we report a new ELISA approach for the detection of specific immunoglobulins (IgGs) against carbohydrates. Two nanometer gold glyconanoparticles bearing oligosaccharide epitopes of HIV or Streptococcus pneumoniae were used as antigens to coat ELISA-plates. A ~3,000-fold improved detection of specific IgGs in mice immunized against S. pneumoniae respect to the ...[more]