Dataset Information

Clinical significance of MYT1L gene polymorphisms in Chinese patients with gastric cancer.

ABSTRACT:

Background

Myelin transcription factor 1 (MYT1) and its homologue MYT1-like (MYT1L) are the two main members of MYT/NZF family transcription factors, which are highly related, share a high degree of identity and show similar regulatory functions in neural development. There are evidences from several cytology experiments showing that MYT1 is associated with carcinoma.

Methodology/principal findings

In the present study, we genotyped 944 surgically resected gastric cancer patients by the SNaPshot method to explore the association of MYT1L rs17039396 polymorphism with survival of gastric cancer in a Chinese population. We found that cardia cancer patients carrying MYT1L rs17039396 GG genotype survived for a significantly shorter time than those carrying the GA genotype. This significance was enhanced in the dominant model (GG vs. GA/AA, log-rank P?=?0.001), suggesting a potential protect role of the variant A allele. Multivariate Cox regression analyses showed that the AG/GG genotypes were associated with a significantly decreased risk of death from gastric cancer (adjusted hazard ratio (HR)?=?0.57, 95% confidence interval (CI)?=?0.40-0.81). Stratification analyses further showed that such protective effect was statistically significant in subgroups of patients with tumor size ?5 cm (adjusted HR?=?0.34, 95%CI?=?0.19-0.64), well-moderate gastric cancer (adjusted HR?=?0.59, 95%CI?=?0.35-0.98), no lymph-node metastasis (adjusted HR?=?0.49, 95%CI?=?0.31-0.76), no distant metastasis (adjusted HR?=?0.59, 95%CI?=?0.41-0.84).

Conclusions/significance

In conclusion, these data represents the first demonstration that MYT1L rs17039396 variants could indentified as a favorable prognostic indicator for gastric cancer, particularly among the cardia gastric cancer. Further validation in other larger studies with different ethnic populations and functional evaluations are needed.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC3756043 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Publications

Clinical significance of MYT1L gene polymorphisms in Chinese patients with gastric cancer.

Zhang Yangmei Y Zhu Haixia H Zhang Xunlei X Gu Dongying D Zhou Xichang X Wang Meilin M Cao Chunxiang C Zhang Xiaojing X Wu Xiaomin X Gong Weida W Tang Yongfei Y Zhou Jianwei J Tang Cuiju C Zhang Zhengdong Z Chen Jinfei J

PloS one 20130828 8

<h4>Background</h4>Myelin transcription factor 1 (MYT1) and its homologue MYT1-like (MYT1L) are the two main members of MYT/NZF family transcription factors, which are highly related, share a high degree of identity and show similar regulatory functions in neural development. There are evidences from several cytology experiments showing that MYT1 is associated with carcinoma.<h4>Methodology/principal findings</h4>In the present study, we genotyped 944 surgically resected gastric cancer patients ...[more]

PMID: 24015200

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Project description:BackgroundThere are a number of studies regarding to the susceptibility of A20 SNPs in rheumatoid arthritis (RA); however, a few of these studies have shown an association between polymorphisms in the A20 gene and RA risk in the Chinese population. The aim of this study was to investigate the characteristics of A20 gene polymorphisms, the association between polymorphisms and clinical significance in Chinese RA patients.MethodsPCR and sequencing were used to identify A20 gene polymorphisms in peripheral blood mononuclear cells (PBMCs) (50 cases), synovial fluid (11 cases) from RA patients and PBMCs from 30 healthy individuals. Quantitative Real-time PCR (qRT-PCR) was used to analyze the A20 mRNA expression in 38 RA patients and 40 healthy individuals. Pearson's Chi square test and two independent-samples Wilcoxon tests were used for statistical analysis.ResultsEight single nucleotide polymorphisms (SNPs) (rs5029937, rs3799491, rs598493, rs2307859, rs146534657, rs2230926, rs661561, and rs582757) were identified in PBMCs of RA patients. One new mutation (14284 T > A) was identified in synovial fluid mononuclear cells from one RA case. rs146534657 was identified for the first time in two RA cases. Patients with rs146534657 (12411 A > G, Asn102Ser) AG genotype or rs2230926 (12486 T > G, Phe127Cys) TG genotype had poor outcome. Significantly lower A20 mRNA expression was found in PBMCs from RA patients compared with healthy individuals (p < 0.001). There was a higher A20 mRNA expression in RA patients with rs2230926 TG genotype and rs146534657 AG genotype (11.56 ± 7.39) than patients with rs2230926 TT genotype and rs146534657 AA genotype (5.63 ± 4.37) (p = 0.031).ConclusionSignificantly lower A20 expression was found in RA patients. The polymorphisms of A20 were characterized in RA patients. We detected rs146534657 for the first time and identified a new A20 mutation (14284 T > A). A20 rs2230926 TG genotype and rs146534657 AG genotype may be related to poor outcome in RA patients.

Dataset Information

Clinical significance of MYT1L gene polymorphisms in Chinese patients with gastric cancer.

Background

Methodology/principal findings

Conclusions/significance

Publications

Clinical significance of MYT1L gene polymorphisms in Chinese patients with gastric cancer.

Similar Datasets

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