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Fibronectin type III domains engineered to bind CD40L: cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of two complexes.

ABSTRACT: Tn3 proteins are a novel class of binding molecules based on the third fibronectin type III domain of human tenascin C. Target-specific Tn3 proteins are selected from combinatorial libraries in which three surface-exposed loops have been diversified. Here, the cocrystallization of two different Tn3 proteins in complex with CD40L, a therapeutic target for immunological disease, is reported. These crystal structures are the first to be reported of Tn3 proteins and will help to reveal how these engineered molecules achieve specific recognition of a cognate target.

SUBMITTER: Oganesyan V

PROVIDER: S-EPMC3758160 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Fibronectin type III domains engineered to bind CD40L: cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of two complexes.

Oganesyan Vaheh V Ferguson Andrew A Grinberg Luba L Wang Lin L Phipps Sandrina S Chacko Benoy B Drabic Stacey S Thisted Thomas T Baca Manuel M

Acta crystallographica. Section F, Structural biology and crystallization communications 20130821 Pt 9

Tn3 proteins are a novel class of binding molecules based on the third fibronectin type III domain of human tenascin C. Target-specific Tn3 proteins are selected from combinatorial libraries in which three surface-exposed loops have been diversified. Here, the cocrystallization of two different Tn3 proteins in complex with CD40L, a therapeutic target for immunological disease, is reported. These crystal structures are the first to be reported of Tn3 proteins and will help to reveal how these eng ...[more]

PMID: 23989160

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