Ontology highlight
ABSTRACT:
SUBMITTER: Hashimoto H
PROVIDER: S-EPMC3758246 | biostudies-literature | 2013 Jul
REPOSITORIES: biostudies-literature
Hashimoto Hideharu H Zhang Xing X Cheng Xiaodong X
DNA repair 20130513 7
The mammalian thymine DNA glycosylase (TDG) excises 5-carboxylcytosine (5caC) when paired with a guanine in a CpG sequence, in addition to mismatched bases. Here we present a complex structure of the human TDG catalytic mutant, asparagine 140 to alanine (N140A), with a 28-base pair DNA containing a G:5caC pair at pH 4.6. TDG interacts with the carboxylate moiety of target nucleotide 5caC using the side chain of asparagine 230 (N230), instead of asparagine 157 (N157) as previously reported. Mutat ...[more]