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Anastomosis of endothelial sprouts forms new vessels in a tissue analogue of angiogenesis.

ABSTRACT: Here we describe a microfluidic device that accurately reproduces the dynamics of vascular anastomosis, the process by which vascular sprouts connect to achieve perfusion during angiogenesis. The micro-device features two parallel endothelial cell-lined vessel analogues separated by a 300 ?m wide collagenous matrix into which the vessels can sprout and form perfused bridging connections. By accurately recapitulating anastomosis in vitro, the device will enable a new generation of studies of the mechanisms of angiogenesis and provide a novel and practical platform for drug screening.

SUBMITTER: Song JW

PROVIDER: S-EPMC3759296 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

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Anastomosis of endothelial sprouts forms new vessels in a tissue analogue of angiogenesis.

Song Jonathan W JW Bazou Despina D Munn Lance L LL

Integrative biology : quantitative biosciences from nano to macro 20120606 8

Here we describe a microfluidic device that accurately reproduces the dynamics of vascular anastomosis, the process by which vascular sprouts connect to achieve perfusion during angiogenesis. The micro-device features two parallel endothelial cell-lined vessel analogues separated by a 300 μm wide collagenous matrix into which the vessels can sprout and form perfused bridging connections. By accurately recapitulating anastomosis in vitro, the device will enable a new generation of studies of the ...[more]

PMID: 22673771

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