Distinct signaling mechanisms regulate migration in unconfined versus confined spaces.

ABSTRACT: Using a microchannel assay, we demonstrate that cells adopt distinct signaling strategies to modulate cell migration in different physical microenvironments. We studied ?4?1 integrin-mediated signaling, which regulates cell migration pertinent to embryonic development, leukocyte trafficking, and melanoma invasion. We show that ?4?1 integrin promotes cell migration through both unconfined and confined spaces. However, unlike unconfined (2D) migration, which depends on enhanced Rac1 activity achieved by preventing ?4/paxillin binding, confined migration requires myosin II-driven contractility, which is increased when Rac1 is inhibited by ?4/paxillin binding. This Rac1-myosin II cross talk mechanism also controls migration of fibroblast-like cells lacking ?4?1 integrin, in which Rac1 and myosin II modulate unconfined and confined migration, respectively. We further demonstrate the distinct roles of myosin II isoforms, MIIA and MIIB, which are primarily required for confined and unconfined migration, respectively. This work provides a paradigm for the plasticity of cells migrating through different physical microenvironments.

SUBMITTER: Hung WC 

PROVIDER: S-EPMC3760608 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature