Isothermal amplification method for next-generation sequencing.
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ABSTRACT:
SUBMITTER: Ma Z
PROVIDER: S-EPMC3761594 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
Publications
Isothermal amplification method for next-generation sequencing.
Ma Zhaochun Z Lee Raymond W RW Li Bin B Kenney Paul P Wang Yufang Y Erikson Jonathan J Goyal Swati S Lao Kaiqin K
Proceedings of the National Academy of Sciences of the United States of America 20130812 35
We report an approach for generating immobilized monoclonal templates for next- generation sequencing applications. Our isothermal amplification method is based on a template walking mechanism using a pair of low-melting temperature (Tm) solid-surface homopolymer primers and a low-Tm solution phase primer. The method can generate more than one billion submicrometer-sized colonies in a single lane of a next-generation sequencing flowchip. An alternative paired-end sequencing method using interstr ...[more]
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