Ontology highlight
ABSTRACT:
SUBMITTER: Lippok S
PROVIDER: S-EPMC3762346 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Lippok Svenja S Obser Tobias T Müller Jochen P JP Stierle Valentin K VK Benoit Martin M Budde Ulrich U Schneppenheim Reinhard R Rädler Joachim O JO
Biophysical journal 20130901 5
Von Willebrand Factor (VWF) is a multimeric protein crucial for hemostasis. Under shear flow, it acts as a mechanosensor responding with a size-dependent globule-stretch transition to increasing shear rates. Here, we quantify for the first time, to our knowledge, the size distribution of recombinant VWF and VWF-eGFP using a multilateral approach that involves quantitative gel analysis, fluorescence correlation spectroscopy, and total internal reflection fluorescence microscopy. We find an expone ...[more]