Carcinogenic adducts induce distinct DNA polymerase binding orientations.
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ABSTRACT:
SUBMITTER: Vrtis KB
PROVIDER: S-EPMC3763543 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Carcinogenic adducts induce distinct DNA polymerase binding orientations.
Vrtis Kyle B KB Markiewicz Radoslaw P RP Romano Louis J LJ Rueda David D
Nucleic acids research 20130628 16
DNA polymerases must accurately replicate DNA to maintain genome integrity. Carcinogenic adducts, such as 2-aminofluorene (AF) and N-acetyl-2-aminofluorene (AAF), covalently bind DNA bases and promote mutagenesis near the adduct site. The mechanism by which carcinogenic adducts inhibit DNA synthesis and cause mutagenesis remains unclear. Here, we measure interactions between a DNA polymerase and carcinogenic DNA adducts in real-time by single-molecule fluorescence. We find the degree to which an ...[more]
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