Dataset Information

First record of Graphosoma inexpectatum (Hemiptera, Pentatomidae, Podopinae) from Turkey with description of the female.

ABSTRACT: Graphosoma inexpectatum Carapezza & Jindra, 2008 is described from Syria, the southern neighbor of Turkey, and is known only from the type locality. The first observation of the species in Turkey dates back to 1995 with two females obtained from the provinces of Gaziantep (?ehitkamil-Aktoprak) and Adana (Pozant?-Bürücek Plateau). These two localities are situated inside the part of the Mediterranean region along the Syrian border. Females of the species, whose original description was based on males, are described here for the first time. A map showing the collecting localities and photographs of the female specimens are given.

SUBMITTER: Fent M

PROVIDER: S-EPMC3764514 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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First record of Graphosoma inexpectatum (Hemiptera, Pentatomidae, Podopinae) from Turkey with description of the female.

Fent Meral M Dursun Ahmet A Tezcan Serdar S

ZooKeys 20130730 319

Graphosoma inexpectatum Carapezza & Jindra, 2008 is described from Syria, the southern neighbor of Turkey, and is known only from the type locality. The first observation of the species in Turkey dates back to 1995 with two females obtained from the provinces of Gaziantep (Şehitkamil-Aktoprak) and Adana (Pozantı-Bürücek Plateau). These two localities are situated inside the part of the Mediterranean region along the Syrian border. Females of the species, whose original description was based on m ...[more]

PMID: 24039511

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Project description:Over the past few years, the use of RNA interference (RNAi) for insect pest management has attracted considerable interest in academia and industry as a pest-specific and environment-friendly strategy for pest control. For the success of this technique, the presence of core RNAi genes and a functional silencing machinery is essential. Therefore, the aim of this study was to test whether the Neotropical brown stinkbug Euschistus heros has the main RNAi core genes and whether the supply of dsRNA could generate an efficient gene silencing response. To do this, total mRNA of all developmental stages was sequenced on an Illumina platform, followed by a de novo assembly, gene annotation and RNAi-related gene identification. Once RNAi-related genes were identified, nuclease activities in hemolymph were investigated through an ex vivo assay. To test the functionality of the siRNA machinery, E. heros adults were microinjected with ~28 ng per mg of insect of a dsRNA targeting the V-ATPase-A gene. Mortality, relative transcript levels of V-ATPase-A, and the expression of the genes involved in the siRNA machinery, Dicer-2 (DCR-2) and Argonaute 2 (AGO-2), were analyzed. Transcriptome sequencing generated more than 126 million sequenced reads, and these were annotated in approximately 80,000 contigs. The search of RNAi-related genes resulted in 47 genes involved in the three major RNAi pathways, with the absence of sid-like homologous. Although ex vivo incubation of dsRNA in E. heros hemolymph showed rapid degradation, there was 35% mortality at 4 days after treatment and a significant reduction in V-ATPase-A gene expression. These results indicated that although sid-like genes are lacking, the dsRNA uptake mechanism was very efficient. Also, 2-fold and 4-fold overexpression of DCR-2 and AGO-2, respectively, after dsRNA supply indicated the activation of the siRNA machinery. Consequently, E. heros has proven to be sensitive to RNAi upon injection of dsRNA into its hemocoel. We believe that this finding together with a publically available transcriptome and the validation of a responsive RNAi machinery provide a starting point for future field applications against one of the most important soybean pests in South America.

Dataset Information

First record of Graphosoma inexpectatum (Hemiptera, Pentatomidae, Podopinae) from Turkey with description of the female.

Publications

First record of Graphosoma inexpectatum (Hemiptera, Pentatomidae, Podopinae) from Turkey with description of the female.

Similar Datasets

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