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Bidirectional processing of pri-miRNAs with branched terminal loops by Arabidopsis Dicer-like1.


ABSTRACT: MicroRNAs (miRNAs) originate from primary transcripts (pri-miRNAs) with characteristic stem-loop structures, and their accurate processing is required for the production of functional miRNAs. Here, using the pri-miR-166 family in Arabidopsis thaliana as a paradigm, we report the crucial role of pri-miRNA terminal loops in miRNA biogenesis. We found that multibranched terminal loops in pri-miR-166s substantially suppress miR-166 expression in vivo. Unlike canonical processing of pri-miRNAs, terminal loop-branched pri-miRNAs can be processed by Dicer-like 1 (DCL1) complexes bidirectionally from base to loop and from loop to base, resulting in productive and abortive processing of miRNAs, respectively. In both cases, DCL1 complexes canonically cut pri-miRNAs at a distance of 16-17 bp from a reference single-stranded loop region. DCL1 also adjusts processing sites toward an internal loop through its helicase domain. These results provide new insight into the poorly understood processing mechanism of pri-miRNAs with complex secondary structures.

SUBMITTER: Zhu H 

PROVIDER: S-EPMC3766402 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Bidirectional processing of pri-miRNAs with branched terminal loops by Arabidopsis Dicer-like1.

Zhu Hongliang H   Zhou Yuyi Y   Castillo-González Claudia C   Castillo-González Claudia C   Lu Amber A   Ge Chunxiao C   Zhao Ying-Tao YT   Duan Liusheng L   Li Zhaohu Z   Axtell Michael J MJ   Wang Xiu-Jie XJ   Zhang Xiuren X  

Nature structural & molecular biology 20130811 9


MicroRNAs (miRNAs) originate from primary transcripts (pri-miRNAs) with characteristic stem-loop structures, and their accurate processing is required for the production of functional miRNAs. Here, using the pri-miR-166 family in Arabidopsis thaliana as a paradigm, we report the crucial role of pri-miRNA terminal loops in miRNA biogenesis. We found that multibranched terminal loops in pri-miR-166s substantially suppress miR-166 expression in vivo. Unlike canonical processing of pri-miRNAs, termi  ...[more]

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