Ontology highlight
ABSTRACT:
SUBMITTER: Qian Z
PROVIDER: S-EPMC3766608 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Qian Zhong Z Zhao Jingjing J Bai Xue X Tong Wei W Chen Zhen Z Wei Hanfu H Wang Quanhui Q Liu Siqi S
BioMed research international 20130824
In the genome of Thermoanaerobacter tengcongensis, three genes belonging to ROK (Repressor, ORF, and Kinase) family are annotated as glucokinases (GLKs). Using enzyme assays, the three GLKs were identified as ATP-dependent GLK (ATP-GLK), ADP-dependent GLK (ADP-GLK), and N-acetyl-glucosamine/mannosamine kinase (glu/man-NacK). The kinetic properties of the three GLKs such as K(m), V(max), optimal pH, and temperature were characterized, demonstrating that these enzymes performed the specific functi ...[more]