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Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression.

ABSTRACT: The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression.

SUBMITTER: Goes LG

PROVIDER: S-EPMC3768373 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Bovine papillomavirus type 4 L1 gene transfection in a Drosophila S2 cell expression system: absence of L1 protein expression.

Góes Luiz Gustavo Bentim LG de Freitas Antonio Carlos AC Ferraz Oilita Pereira OP Rieger Tania Tassinari TT Dos Santos José Ferreira JF Pereira Alexandre A Beçak Willy W Lindsey Charles J CJ de Cassia Stocco Rita R

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20080101 1

The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. BPV protein L1 gene transfection in the Drosophila melanogaster S2 cell expression system failed to produce L1 protein notwithstanding correct L1 gene insertion. Severe genetic inbalance in the host cell line, including cytogenetic alterations, may account for the lack of protein expression. ...[more]

PMID: 24031166

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