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Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems.

ABSTRACT: Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experiment was conducted for testing validity using 40 animals. In conclusion, the obtained results of the N-PCR optimization and validation allow us to recommend this test as a routine monitoring diagnostic method for Mycoplasma hyopneumoniae infection in swine herds.

SUBMITTER: Yamaguti M

PROVIDER: S-EPMC3768421 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Detection of Mycoplasma hyopneumoniae by polymerase chain reaction in swine presenting respiratory problems.

Yamaguti M M Muller E E EE Piffer A I AI Kich J D JD Klein C S CS Kuchiishi S S SS

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20080701 3

Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experim ...[more]

PMID: 24031248

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