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Selective fluorogenic chemosensors for distinct classes of nucleases.


ABSTRACT: NUCLEASE SENSOR TRIO: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3'-exonucleases, 5'-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.

SUBMITTER: Jung JW 

PROVIDER: S-EPMC3770146 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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Selective fluorogenic chemosensors for distinct classes of nucleases.

Jung Jong-Wha JW   Edwards Sarah K SK   Kool Eric T ET  

Chembiochem : a European journal of chemical biology 20130201 4


NUCLEASE SENSOR TRIO: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3'-exonucleases, 5'-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media. ...[more]

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