Selective fluorogenic chemosensors for distinct classes of nucleases.

ABSTRACT: NUCLEASE SENSOR TRIO: Fluorogenic DNA sensors were developed for distinct classes of nucleases: 3'-exonucleases, 5'-exonucleases, and endonucleases. The highly selective sensors, built from very small modified DNA oligomers containing the unnatural fluorescent base pyrene, and employing thymine as a quencher, were found to function in a variety of complex biological media.

SUBMITTER: Jung JW 

PROVIDER: S-EPMC3770146 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature