Ontology highlight
ABSTRACT:
SUBMITTER: Sun L
PROVIDER: S-EPMC3770260 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Sun Liangliang L Knierman Michael D MD Zhu Guijie G Dovichi Norman J NJ
Analytical chemistry 20130607 12
Capillary zone electrophoresis (CZE)-electrospray ionization tandem mass spectrometry (ESI-MS/MS) was applied for rapid top-down intact protein characterization. A mixture containing four model proteins (cytochrome c, myoglobin, bovine serum albumin (BSA), and β-casein) was used as the sample. The CZE-ESI-MS system was first evaluated with the mixture. The four model proteins and five impurities were baseline-separated within 12 min. The limits of detection [signal-to-noise ratio (S/N) = 3] of t ...[more]